Session 3, Abstract 13

A STUDY OF GENES EXPRESSED IN IMMUNOGLOBULIN M B CELLS FROM THE ZEBRAFISH KIDNEY​.

Christiana Crook*, (Dawne Page), Point Loma Nazarene University, Dept. of Biology, 3900 Lomaland Drive, San Diego, CA 92106.

Bony fish are the most primitive vertebrates to possess an adaptive immune system. The zebrafish (Danio rerio) is one example of these bony fish, which means they have an immune system similar to that of a human (composed of both an innate and an adaptive immune system). As such, zebrafish are an excellent model for studying the evolution of the adaptive immune system. The immune system is composed of many different types of cells; one of these populations is known as B cells. B cells can further be classified into developing, mature, plasma, or memory B cells. Each subset of B cells has unique characteristics and functions within the immune system. It was not known what classes of B cells were present in zebrafish kidneys (the location where B cell development occurs) in certain transgenic models. This knowledge could further increase the use of zebrafish as an animal model by allowing researchers to know what types of B cells were being used in an immune response, and how this could affect the observed results. This study used double transgenic fluorescent zebrafish, where the two fluorophores used labeled and differentiated various cell populations. Cell samples from the kidneys of zebrafish were analyzed to determine if cells that expressed both fluorophores were a pure population of plasma B cells. It was found that populations of double-fluorescent cells, which consisted of either IgMhi cells or IgM+BLIMP+ cells, are composed of both mature and plasma B cells.

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